Chromatin Immunoprecipitation:Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Primary antibodies: mouse anti-Cre Recombinase Merck millipore MAB3120 JC1631396 1:500 - Manufacturer:Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications. mouse anti-S-100b SIGMA S2532 048m4858v 1:500 -Manufacturer:These antibodies have been verified by Relative Expression to confirm specificity to S100B. mouse anti-NeuN Merck millipore MAB377 1991263 1:500 - Manufacturer: it detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used All antibodies (provider, ordering numbers and dilution) used in this study are listed in supplementary Table 3. guine pig anti-Iba1 Synaptic System 234004 2-28 1:500 mouse anti-Cre Recombinase Merk millipore MAB3120 JC1631396 1:500 mouse anti-S-100b SIGMA S2532 048m4858v 1:500 mouse anti-NeuN Merk millipore MAB377 1991263 1:500 mouse anti-glutamine synthetase Merk millipore MAB302 2676275 1:500 mouse anti-Olig2 Merk millipore MABN50 3421971 1:500 rabbit anti-glutamine synthetase Abcam ab 73593 gr3200078-1 1:500 rabbit anti-S100b Synaptic system S287003 1-5 1:500 rabbit anti-Olig2 NovusBio NBP1-28667 DF1210 1:100 rabbit Anti-Tyrosine Hydroxylase Merk millipore AB152 3114503 1:200 chicken anti-S100b Synaptic system 287006 287006/1-4 1:500 goat anti-TdTomato BioSource cat. n. MBS448092 lot: 0081191218 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405 Thermofisher Cat # A48255 51912A 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-32723 TC252656 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21052 1906490 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-11034 SH251139 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21071 1932492 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermofisher Cat # A-11073 1458631 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21105 514962 1:500 Validation All antibodies used in this study are from commercial suppliers (see notes above for each antibody) that have verified the specificity of the antibodies.
Article Title: Adult Mammalian Forebrain Ependymal and Subependymal Cells Demonstrate Proliferative Potential, but only Subependymal Cells Have Neural Stem Cell Characteristics
Article Snippet: The fixative was then washed with 0.1 M PBS, and the tissue was frozen at 220°C and subsequently processed for immunocytochemistry for both BrdU (rat monoclonal anti-BrdU, 1:100; SeraLab, London, UK) and S-100b (mouse anti-S-100b, 1:500; Sigma), which labels ependymal cells but not subependymal cells (Craig et al., 1996).
Article Title: Environmental signals elicit multiple responses in dorsal telencephalic progenitors by threshold-dependent mechanisms.
Article Snippet: D ow nloaded from https://academ ic.oup.com /cercor/article/16/suppl_1/i74/270139 by guest on 04 August 2024 Biosciences/Pharmingen), mouse anti-S-100b (Sigma), mouse anti--glial fibrillary acidic protein (Sigma), rabbit anti-GABA (Sigma), mouse anti-proliferating cell nuclear antigen (PCNA) (Sigma), mouse anti-TuJ1 (Covance Research Products), mouse or rabbit anti-b-galactosidase (Promega, CortexBiochem),mouseor rabbit anti-GFP (Molecular Probes), rabbit anti--signal transducer and activator of transcription 3 (STAT3) (Cell Signaling Technology), mouse anti--glutamate decarboxylase 65 (GAD65) (DSHB or Chemicon), rabbit anti--dopamine and cyclic AMP-regulated phosphoprotein 32 (DARPP32) (Cell Signaling Technology), mouse antiglutamate (Chemicon), rabbit anti-glutamate (Sigma), and rabbit anti-NG2 (Chemicon).
Flow Cytometry:Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Primary antibodies: mouse anti-Cre Recombinase Merck millipore MAB3120 JC1631396 1:500 - Manufacturer:Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications. mouse anti-S-100b SIGMA S2532 048m4858v 1:500 -Manufacturer:These antibodies have been verified by Relative Expression to confirm specificity to S100B. mouse anti-NeuN Merck millipore MAB377 1991263 1:500 - Manufacturer: it detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used All antibodies (provider, ordering numbers and dilution) used in this study are listed in supplementary Table 3. guine pig anti-Iba1 Synaptic System 234004 2-28 1:500 mouse anti-Cre Recombinase Merk millipore MAB3120 JC1631396 1:500 mouse anti-S-100b SIGMA S2532 048m4858v 1:500 mouse anti-NeuN Merk millipore MAB377 1991263 1:500 mouse anti-glutamine synthetase Merk millipore MAB302 2676275 1:500 mouse anti-Olig2 Merk millipore MABN50 3421971 1:500 rabbit anti-glutamine synthetase Abcam ab 73593 gr3200078-1 1:500 rabbit anti-S100b Synaptic system S287003 1-5 1:500 rabbit anti-Olig2 NovusBio NBP1-28667 DF1210 1:100 rabbit Anti-Tyrosine Hydroxylase Merk millipore AB152 3114503 1:200 chicken anti-S100b Synaptic system 287006 287006/1-4 1:500 goat anti-TdTomato BioSource cat. n. MBS448092 lot: 0081191218 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405 Thermofisher Cat # A48255 51912A 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-32723 TC252656 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21052 1906490 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-11034 SH251139 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21071 1932492 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermofisher Cat # A-11073 1458631 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21105 514962 1:500 Validation All antibodies used in this study are from commercial suppliers (see notes above for each antibody) that have verified the specificity of the antibodies.
Article Title: Adult Mammalian Forebrain Ependymal and Subependymal Cells Demonstrate Proliferative Potential, but only Subependymal Cells Have Neural Stem Cell Characteristics
Article Snippet: The fixative was then washed with 0.1 M PBS, and the tissue was frozen at 220°C and subsequently processed for immunocytochemistry for both BrdU (rat monoclonal anti-BrdU, 1:100; SeraLab, London, UK) and S-100b (mouse anti-S-100b, 1:500; Sigma), which labels ependymal cells but not subependymal cells (Craig et al., 1996).
Article Title: Environmental signals elicit multiple responses in dorsal telencephalic progenitors by threshold-dependent mechanisms.
Article Snippet: D ow nloaded from https://academ ic.oup.com /cercor/article/16/suppl_1/i74/270139 by guest on 04 August 2024 Biosciences/Pharmingen), mouse anti-S-100b (Sigma), mouse anti--glial fibrillary acidic protein (Sigma), rabbit anti-GABA (Sigma), mouse anti-proliferating cell nuclear antigen (PCNA) (Sigma), mouse anti-TuJ1 (Covance Research Products), mouse or rabbit anti-b-galactosidase (Promega, CortexBiochem),mouseor rabbit anti-GFP (Molecular Probes), rabbit anti--signal transducer and activator of transcription 3 (STAT3) (Cell Signaling Technology), mouse anti--glutamate decarboxylase 65 (GAD65) (DSHB or Chemicon), rabbit anti--dopamine and cyclic AMP-regulated phosphoprotein 32 (DARPP32) (Cell Signaling Technology), mouse antiglutamate (Chemicon), rabbit anti-glutamate (Sigma), and rabbit anti-NG2 (Chemicon).
Magnetic Resonance Imaging:Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Primary antibodies: mouse anti-Cre Recombinase Merck millipore MAB3120 JC1631396 1:500 - Manufacturer:Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications. mouse anti-S-100b SIGMA S2532 048m4858v 1:500 -Manufacturer:These antibodies have been verified by Relative Expression to confirm specificity to S100B. mouse anti-NeuN Merck millipore MAB377 1991263 1:500 - Manufacturer: it detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used All antibodies (provider, ordering numbers and dilution) used in this study are listed in supplementary Table 3. guine pig anti-Iba1 Synaptic System 234004 2-28 1:500 mouse anti-Cre Recombinase Merk millipore MAB3120 JC1631396 1:500 mouse anti-S-100b SIGMA S2532 048m4858v 1:500 mouse anti-NeuN Merk millipore MAB377 1991263 1:500 mouse anti-glutamine synthetase Merk millipore MAB302 2676275 1:500 mouse anti-Olig2 Merk millipore MABN50 3421971 1:500 rabbit anti-glutamine synthetase Abcam ab 73593 gr3200078-1 1:500 rabbit anti-S100b Synaptic system S287003 1-5 1:500 rabbit anti-Olig2 NovusBio NBP1-28667 DF1210 1:100 rabbit Anti-Tyrosine Hydroxylase Merk millipore AB152 3114503 1:200 chicken anti-S100b Synaptic system 287006 287006/1-4 1:500 goat anti-TdTomato BioSource cat. n. MBS448092 lot: 0081191218 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405 Thermofisher Cat # A48255 51912A 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-32723 TC252656 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21052 1906490 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-11034 SH251139 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21071 1932492 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermofisher Cat # A-11073 1458631 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21105 514962 1:500 Validation All antibodies used in this study are from commercial suppliers (see notes above for each antibody) that have verified the specificity of the antibodies.
Article Title: Adult Mammalian Forebrain Ependymal and Subependymal Cells Demonstrate Proliferative Potential, but only Subependymal Cells Have Neural Stem Cell Characteristics
Article Snippet: The fixative was then washed with 0.1 M PBS, and the tissue was frozen at 220°C and subsequently processed for immunocytochemistry for both BrdU (rat monoclonal anti-BrdU, 1:100; SeraLab, London, UK) and S-100b (mouse anti-S-100b, 1:500; Sigma), which labels ependymal cells but not subependymal cells (Craig et al., 1996).
Article Title: Environmental signals elicit multiple responses in dorsal telencephalic progenitors by threshold-dependent mechanisms.
Article Snippet: D ow nloaded from https://academ ic.oup.com /cercor/article/16/suppl_1/i74/270139 by guest on 04 August 2024 Biosciences/Pharmingen), mouse anti-S-100b (Sigma), mouse anti--glial fibrillary acidic protein (Sigma), rabbit anti-GABA (Sigma), mouse anti-proliferating cell nuclear antigen (PCNA) (Sigma), mouse anti-TuJ1 (Covance Research Products), mouse or rabbit anti-b-galactosidase (Promega, CortexBiochem),mouseor rabbit anti-GFP (Molecular Probes), rabbit anti--signal transducer and activator of transcription 3 (STAT3) (Cell Signaling Technology), mouse anti--glutamate decarboxylase 65 (GAD65) (DSHB or Chemicon), rabbit anti--dopamine and cyclic AMP-regulated phosphoprotein 32 (DARPP32) (Cell Signaling Technology), mouse antiglutamate (Chemicon), rabbit anti-glutamate (Sigma), and rabbit anti-NG2 (Chemicon).
Enzyme-linked Immunosorbent Assay:Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Primary antibodies: mouse anti-Cre Recombinase Merck millipore MAB3120 JC1631396 1:500 - Manufacturer:Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications. mouse anti-S-100b SIGMA S2532 048m4858v 1:500 -Manufacturer:These antibodies have been verified by Relative Expression to confirm specificity to S100B. mouse anti-NeuN Merck millipore MAB377 1991263 1:500 - Manufacturer: it detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used All antibodies (provider, ordering numbers and dilution) used in this study are listed in supplementary Table 3. guine pig anti-Iba1 Synaptic System 234004 2-28 1:500 mouse anti-Cre Recombinase Merk millipore MAB3120 JC1631396 1:500 mouse anti-S-100b SIGMA S2532 048m4858v 1:500 mouse anti-NeuN Merk millipore MAB377 1991263 1:500 mouse anti-glutamine synthetase Merk millipore MAB302 2676275 1:500 mouse anti-Olig2 Merk millipore MABN50 3421971 1:500 rabbit anti-glutamine synthetase Abcam ab 73593 gr3200078-1 1:500 rabbit anti-S100b Synaptic system S287003 1-5 1:500 rabbit anti-Olig2 NovusBio NBP1-28667 DF1210 1:100 rabbit Anti-Tyrosine Hydroxylase Merk millipore AB152 3114503 1:200 chicken anti-S100b Synaptic system 287006 287006/1-4 1:500 goat anti-TdTomato BioSource cat. n. MBS448092 lot: 0081191218 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405 Thermofisher Cat # A48255 51912A 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-32723 TC252656 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21052 1906490 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-11034 SH251139 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21071 1932492 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermofisher Cat # A-11073 1458631 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21105 514962 1:500 Validation All antibodies used in this study are from commercial suppliers (see notes above for each antibody) that have verified the specificity of the antibodies.
Article Title: Adult Mammalian Forebrain Ependymal and Subependymal Cells Demonstrate Proliferative Potential, but only Subependymal Cells Have Neural Stem Cell Characteristics
Article Snippet: The fixative was then washed with 0.1 M PBS, and the tissue was frozen at 220°C and subsequently processed for immunocytochemistry for both BrdU (rat monoclonal anti-BrdU, 1:100; SeraLab, London, UK) and S-100b (mouse anti-S-100b, 1:500; Sigma), which labels ependymal cells but not subependymal cells (Craig et al., 1996).
Article Title: Environmental signals elicit multiple responses in dorsal telencephalic progenitors by threshold-dependent mechanisms.
Article Snippet: D ow nloaded from https://academ ic.oup.com /cercor/article/16/suppl_1/i74/270139 by guest on 04 August 2024 Biosciences/Pharmingen), mouse anti-S-100b (Sigma), mouse anti--glial fibrillary acidic protein (Sigma), rabbit anti-GABA (Sigma), mouse anti-proliferating cell nuclear antigen (PCNA) (Sigma), mouse anti-TuJ1 (Covance Research Products), mouse or rabbit anti-b-galactosidase (Promega, CortexBiochem),mouseor rabbit anti-GFP (Molecular Probes), rabbit anti--signal transducer and activator of transcription 3 (STAT3) (Cell Signaling Technology), mouse anti--glutamate decarboxylase 65 (GAD65) (DSHB or Chemicon), rabbit anti--dopamine and cyclic AMP-regulated phosphoprotein 32 (DARPP32) (Cell Signaling Technology), mouse antiglutamate (Chemicon), rabbit anti-glutamate (Sigma), and rabbit anti-NG2 (Chemicon).
Western Blot:Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Primary antibodies: mouse anti-Cre Recombinase Merck millipore MAB3120 JC1631396 1:500 - Manufacturer:Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications. mouse anti-S-100b SIGMA S2532 048m4858v 1:500 -Manufacturer:These antibodies have been verified by Relative Expression to confirm specificity to S100B. mouse anti-NeuN Merck millipore MAB377 1991263 1:500 - Manufacturer: it detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used All antibodies (provider, ordering numbers and dilution) used in this study are listed in supplementary Table 3. guine pig anti-Iba1 Synaptic System 234004 2-28 1:500 mouse anti-Cre Recombinase Merk millipore MAB3120 JC1631396 1:500 mouse anti-S-100b SIGMA S2532 048m4858v 1:500 mouse anti-NeuN Merk millipore MAB377 1991263 1:500 mouse anti-glutamine synthetase Merk millipore MAB302 2676275 1:500 mouse anti-Olig2 Merk millipore MABN50 3421971 1:500 rabbit anti-glutamine synthetase Abcam ab 73593 gr3200078-1 1:500 rabbit anti-S100b Synaptic system S287003 1-5 1:500 rabbit anti-Olig2 NovusBio NBP1-28667 DF1210 1:100 rabbit Anti-Tyrosine Hydroxylase Merk millipore AB152 3114503 1:200 chicken anti-S100b Synaptic system 287006 287006/1-4 1:500 goat anti-TdTomato BioSource cat. n. MBS448092 lot: 0081191218 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405 Thermofisher Cat # A48255 51912A 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-32723 TC252656 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21052 1906490 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-11034 SH251139 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21071 1932492 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermofisher Cat # A-11073 1458631 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21105 514962 1:500 Validation All antibodies used in this study are from commercial suppliers (see notes above for each antibody) that have verified the specificity of the antibodies.
Article Title: Adult Mammalian Forebrain Ependymal and Subependymal Cells Demonstrate Proliferative Potential, but only Subependymal Cells Have Neural Stem Cell Characteristics
Article Snippet: The fixative was then washed with 0.1 M PBS, and the tissue was frozen at 220°C and subsequently processed for immunocytochemistry for both BrdU (rat monoclonal anti-BrdU, 1:100; SeraLab, London, UK) and S-100b (mouse anti-S-100b, 1:500; Sigma), which labels ependymal cells but not subependymal cells (Craig et al., 1996).
Article Title: Environmental signals elicit multiple responses in dorsal telencephalic progenitors by threshold-dependent mechanisms.
Article Snippet: D ow nloaded from https://academ ic.oup.com /cercor/article/16/suppl_1/i74/270139 by guest on 04 August 2024 Biosciences/Pharmingen), mouse anti-S-100b (Sigma), mouse anti--glial fibrillary acidic protein (Sigma), rabbit anti-GABA (Sigma), mouse anti-proliferating cell nuclear antigen (PCNA) (Sigma), mouse anti-TuJ1 (Covance Research Products), mouse or rabbit anti-b-galactosidase (Promega, CortexBiochem),mouseor rabbit anti-GFP (Molecular Probes), rabbit anti--signal transducer and activator of transcription 3 (STAT3) (Cell Signaling Technology), mouse anti--glutamate decarboxylase 65 (GAD65) (DSHB or Chemicon), rabbit anti--dopamine and cyclic AMP-regulated phosphoprotein 32 (DARPP32) (Cell Signaling Technology), mouse antiglutamate (Chemicon), rabbit anti-glutamate (Sigma), and rabbit anti-NG2 (Chemicon).
Expressing:Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Primary antibodies: mouse anti-Cre Recombinase Merck millipore MAB3120 JC1631396 1:500 - Manufacturer:Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications. mouse anti-S-100b SIGMA S2532 048m4858v 1:500 -Manufacturer:These antibodies have been verified by Relative Expression to confirm specificity to S100B. mouse anti-NeuN Merck millipore MAB377 1991263 1:500 - Manufacturer: it detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
Article Title: Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used All antibodies (provider, ordering numbers and dilution) used in this study are listed in supplementary Table 3. guine pig anti-Iba1 Synaptic System 234004 2-28 1:500 mouse anti-Cre Recombinase Merk millipore MAB3120 JC1631396 1:500 mouse anti-S-100b SIGMA S2532 048m4858v 1:500 mouse anti-NeuN Merk millipore MAB377 1991263 1:500 mouse anti-glutamine synthetase Merk millipore MAB302 2676275 1:500 mouse anti-Olig2 Merk millipore MABN50 3421971 1:500 rabbit anti-glutamine synthetase Abcam ab 73593 gr3200078-1 1:500 rabbit anti-S100b Synaptic system S287003 1-5 1:500 rabbit anti-Olig2 NovusBio NBP1-28667 DF1210 1:100 rabbit Anti-Tyrosine Hydroxylase Merk millipore AB152 3114503 1:200 chicken anti-S100b Synaptic system 287006 287006/1-4 1:500 goat anti-TdTomato BioSource cat. n. MBS448092 lot: 0081191218 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 405 Thermofisher Cat # A48255 51912A 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-32723 TC252656 1:500 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21052 1906490 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 Thermofisher Cat # A-11034 SH251139 1:500 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21071 1932492 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermofisher Cat # A-11073 1458631 1:500 Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 Thermofisher Cat # A-21105 514962 1:500 Validation All antibodies used in this study are from commercial suppliers (see notes above for each antibody) that have verified the specificity of the antibodies.
Article Title: Adult Mammalian Forebrain Ependymal and Subependymal Cells Demonstrate Proliferative Potential, but only Subependymal Cells Have Neural Stem Cell Characteristics
Article Snippet: The fixative was then washed with 0.1 M PBS, and the tissue was frozen at 220°C and subsequently processed for immunocytochemistry for both BrdU (rat monoclonal anti-BrdU, 1:100; SeraLab, London, UK) and S-100b (mouse anti-S-100b, 1:500; Sigma), which labels ependymal cells but not subependymal cells (Craig et al., 1996).
Article Title: Environmental signals elicit multiple responses in dorsal telencephalic progenitors by threshold-dependent mechanisms.
Article Snippet: D ow nloaded from https://academ ic.oup.com /cercor/article/16/suppl_1/i74/270139 by guest on 04 August 2024 Biosciences/Pharmingen), mouse anti-S-100b (Sigma), mouse anti--glial fibrillary acidic protein (Sigma), rabbit anti-GABA (Sigma), mouse anti-proliferating cell nuclear antigen (PCNA) (Sigma), mouse anti-TuJ1 (Covance Research Products), mouse or rabbit anti-b-galactosidase (Promega, CortexBiochem),mouseor rabbit anti-GFP (Molecular Probes), rabbit anti--signal transducer and activator of transcription 3 (STAT3) (Cell Signaling Technology), mouse anti--glutamate decarboxylase 65 (GAD65) (DSHB or Chemicon), rabbit anti--dopamine and cyclic AMP-regulated phosphoprotein 32 (DARPP32) (Cell Signaling Technology), mouse antiglutamate (Chemicon), rabbit anti-glutamate (Sigma), and rabbit anti-NG2 (Chemicon).
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Millipore is a verified supplier 